The Postnova AF2000 MultiFlow FFF Series is the first professional modular Flow FFF platform, integrated by the NovaFFF software which runs the entire system from autosampler to detectors. Built on three decades of FFF expertise, it offers superior flexibility, robustness, and performance, setting a new standard in Flow FFF science.

Based on the Flow FFF principle, the AF2000 uses a crossflow field to separate samples by Dynamic Diffusion according to molar mass or particle size. It supports different eluents, various temperature conditions, and multiple channel dimensions — running both planar Asymmetric Flow FFF (AF4) and circular HollowFiber Flow FFF (HF5) channels.

Applications:

• Biopharmaceuticals

• Food-Agro-Cosmetics

• Environmental

• Chemicals

• Nanotechnology

Ideal for characterizing proteins, antibodies, aggregates, vaccines, liposomes, nanoparticles, and natural or synthetic macromolecules, the AF2000 serves as a universal separator for a broad range of analyte systems.

Two versions available:

• AF2000 AT – Non-temperature controlled, economical version for general research use, available in aqueous metal-free, stainless steel, and multisolvent variants

• AF2000 MT – Temperature controlled version for advanced research, product development, manufacturing, and quality control; temperature control extends to the entire system, including transfer capillaries

Compatible detectors: UV-Absorbance (UV), Refractive Index (RI), Multi-Angle Light Scattering (MALS), Dynamic Light Scattering (DLS), and Inductively Coupled Plasma Mass Spectroscopy (ICP-MS)

The Postnova CF2000 Centrifugal FFF Series is the first professional modular Centrifugal FFF system, controlled by the NovaFFF software which runs the entire system from autosampler to detectors. Built on three decades of FFF expertise, it offers greater flexibility, robustness, and performance than traditional particle sizer systems, enabling high resolution particle separation and sizing simultaneously.

The CF2000 offers a broad separation range, separating smaller species such as proteins and polymers from larger particles in a single run with high resolution. It is ideal for nanoparticles and also suitable for the microparticle range. Unlike traditional batch-mode particle sizing techniques, Centrifugal FFF physically separates each particle fraction prior to sizing — avoiding low size resolution and the discrimination or underestimation of smaller particles by larger ones. As with Flow FFF, no special sample treatment is required; samples can be injected directly without filtration.

Typical samples:

• Latex, silica, TiO2, ZnO, Ag and Au particles

• Sediment and organic/inorganic particles from surface, ground, and waste waters

Based on the Centrifugal FFF principle, the system uses a centrifugal field to separate particles by Dynamic Diffusion according to both particle size and density — a unique feature that allows separation of different particle materials with the same size. Separation can be further optimized using different eluents and temperature programs.

Applications:

• Environmental

• Food-Agro-Cosmetics

• Nanomaterials

Compatible detectors: Refractive Index (RI), Multi-Angle Light Scattering (MALS), and Dynamic Light Scattering (DLS)

The Postnova SC2000 Modular SEC system is the first truly modular multi-detector SEC/GPC platform, offering unrivalled flexibility for a wide range of applications in the separation and characterization of polymers, proteins, and polysaccharides.

The system incorporates state-of-the-art detection technologies, including light scattering (Multi-Angle Light Scattering, MALS) for absolute molecular weight determination and viscosity detection for structural and conformational data. A broad range of additional detectors and accessories can be combined to build a system uniquely tailored to specific application requirements, with modularity that allows easy future expansion.

The SC2000 can also be expanded to include FFF modules, enabling both SEC and FFF separations to be carried out on the same sample within one instrument platform.

Drawing on over three decades of leadership in separation science, Postnova has designed a solid, stable SEC platform to help users achieve maximum success with their applications.

The Postnova PN3600 Series of Multi-Angle Light Scattering detectors was developed specifically for coupling with Postnova Field-Flow Fractionation systems. Based on over two decades of experience in FFF-MALS hyphenation, the PN3621 is the top model of the series, offering the most flexible and powerful MALS solution available, with unique technical features designed for the highest sensitivity, precision, and flexibility.

Maximum number of angles
The PN3621 offers 21 full working angles in aqueous and organic solvents — the broadest angular range of any commercial light scattering instrument available today. This enables the highest precision in molecular weight and particle size determination, particularly for high molar mass polymers, particles, and protein aggregates.

Unique low angle range
Precision depends not only on the total number of angles, but especially on the number of working low angles — critical for branched polymers, high molar mass macromolecules, protein aggregates, and particles. The PN3621 provides a complete set of stable low angles starting at 7°, 12°, 20°, and 28°.

Vertical flow cell
Since MALS is often used to measure larger, fragile sample species, gentle sample treatment is essential to avoid shear forces, chain degradation, conformational changes, or loss of bioactivity. The PN3621's vertical flow cell lets samples pass through without alteration, avoiding the internal obstacles, edges, and dead volumes that can cause aggregation, adsorption, or sedimentation in conventional cells.

Advanced electro-optics
The PN3621 uses the latest opto-electronic and laser designs, with digital signal processing for each of the 21 angles and a true 24-bit resolution AD system for a broad dynamic signal range. Up to 4 external analogue signals can be read in for added flexibility. The instrument is compact, lightweight, and has a small footprint, making it easy to use and transport.

Applications:

• Biotechnology – alginates, carrageenans, hyaluronic acids, cell organelles, exosomes

• Biopharmaceutical – peptide, protein, antibody, virus aggregates and conjugates

• Food and Agro Science – starches, pectins, polysaccharides, proteins, casein micelles

• Polymer Science – rubbers, latex dispersions, polyolefins, polyelectrolytes

• Environmental Research – humic-fulvic colloids, iron oxides, clays, silica particles

• Nanotechnology – TiO2, CNT, C60, latex, ZnO and silica nanoparticles

The Postnova PN3310 Viscometer is a state-of-the-art intrinsic viscosity detector for Field-Flow Fractionation (FFF) and Size Exclusion Chromatography (GPC/SEC). Based on the proven Wheatstone-Bridge design, it measures the intrinsic viscosity of macromolecules in solution and features the modern, compact Postnova FFF detector design.

The PN3310 delivers outstanding performance with highly sensitive pressure transducers. Its Pressure Sensor Protection (PSP) system automatically prevents critical overpressure on the transducers, while the instrument offers excellent baseline stability with extremely low drift and a superior signal-to-noise ratio. Up to four analog signals from external detectors (e.g. RI, UV, Fluorescence) can be connected. The detector is fully controlled by the NovaMALS software, which can also integrate additional detectors such as Multi-Angle Light Scattering (MALS), Refractive Index (RI), and UV Absorbance.

Modular detection systems
Combined with the Postnova PN3621 MALS detector and PN3150 RI detector, a modular, flexible, and compact Triple Detection System can be configured. Adding a PN3211 Dual Wavelength UV or PN3242 Diode Array UV as a second concentration detector allows a Quadruple Detection System to be set up.

Molecular size and structure
Using the PN3310 together with a concentration detector (RI, UV) and a light scattering detector (MALS), both the molecular size (hydrodynamic, gyration) and structure (shape, branching) of macromolecules can be determined. With or without an added light scattering detector, the Mark-Houwink plot (molecular weight vs. intrinsic viscosity) can be derived using the NovaMALS software — one of the most powerful structure plots in macromolecular analysis, providing extensive information on the size, shape, structure, and molecular weight of macromolecules in solution.